The information you provide should typically answer the following questions: 

The materials and methods section should give enough detail so that the experiment can be repeated in another laboratory. Cover all the experimental aspects but avoid repetition. Do not comment on your observations or measurements in the methods stage. You should do this in the results stage. It is usual to integrate your description of the materials with your recount of the methods. However, in some experiments, you may need to describe some of your materials separately.

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